兔抗PRKACA多克隆抗体

Background:

This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms.

Applications:

ELISA, WB, IHC

Name of antibody:

PRKACA

Immunogen:

Synthetic peptide of human PRKACA

Full name:

protein kinase cAMP-activated catalytic subunit alpha

Synonyms:

PKACA; PPNAD4

SwissProt:

P17612

ELISA Recommended dilution:

5000-10000

IHC positive control:

Human liver cancer and human brain

IHC Recommend dilution:

25-100

WB Predicted band size:

41 kDa

WB Positive control:

Human testis tissue lysate

WB Recommended dilution:

200-1000