兔抗PDHA1多克隆抗体

Background:

The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Applications:

ELISA, WB, IHC

Name of antibody:

PDHA1

Immunogen:

Fusion protein of human PDHA1

Full name:

pyruvate dehydrogenase E1 alpha 1 subunit

Synonyms:

PDHA; PDHAD; PHE1A; PDHCE1A

SwissProt:

P08559

ELISA Recommended dilution:

5000-10000

IHC positive control:

Human cervical cancer and Human tonsil

IHC Recommend dilution:

50-300

WB Predicted band size:

43 kDa

WB Positive control:

Mouse brain tissue, HepG2, Hela, A549 cell, Mouse skeletal muscle tissue, NIH/3T3 cell lysates

WB Recommended dilution:

1000-5000