兔抗PTEN (Phospho-Ser380 Thr382 Thr383)多克隆抗体

Background:

Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue.

Applications:

WB, IHC, IF

Name of antibody:

PTEN (Phospho-Ser380/Thr382/Thr383)

Immunogen:

Synthetic peptide of human PTEN (Phospho-Ser380/Thr382/Thr383)

Full name:

phosphatase and tensin homolog (Phospho-Ser380/Thr382/Thr383)

Synonyms：

BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del

SwissProt:

P60484

IHC positive control:

Human breast carcinoma

IHC Recommend dilution:

50-100

WB Predicted band size:

54 kDa

WB Positive control:

Hela cells

WB Recommended dilution:

500-1000

IF Positive control:

Hela cells

IF Recommended dilution

100-200