手机扫码访问本站
微信咨询
技术规格
Background:
The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.
Applications:
ELISA, WB
Name of antibody:
RAD17
Immunogen:
Fusion protein of human RAD17
Full name:
RAD17 checkpoint clamp loader component
Synonyms:
CCYC; R24L; RAD24; HRAD17; RAD17SP
SwissProt:
O75943
ELISA Recommended dilution:
1000-2000
WB Predicted band size:
77 kDa
WB Positive control:
Lovo cells
WB Recommended dilution:
200-1000