兔抗MRPL32多克隆抗体

Background:

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp.

Applications:

WB, IF

Name of antibody:

MRPL32

Immunogen:

Synthesized peptide derived from internal of human MRPL32.

Full name:

mitochondrial ribosomal protein L32

Synonyms：

L32mt; HSPC283; MRP-L32; bMRP-59b

SwissProt:

Q9BYC8

WB Predicted band size:

21 kDa

WB Positive control:

Jurkat cells, MCF-7 cells and HepG2 cells lysates

WB Recommended dilution:

500-3000

IF positive control:

A549 cells

IF Recommend dilution:

100-500