兔抗RPA2(Phospho-Thr21) 多克隆抗体

Background:

Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage. Ref.9 Ref.14 Ref.15 Ref.16 Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

Applications:

WB

Name of antibody:

RPA2(Phospho-Thr21)

Immunogen:

Peptide sequence around phosphorylation site of Threonine 21 (G-Y-T(p)-Q-S) derived from Human RFA2.

Full name:

replication protein A2, 32kDa

Synonyms：

REPA2; RPA32; RP-A p32; RP-A p34

SwissProt:

P15927

WB Predicted band size:

29 kDa

WB Positive control:

Rat heart tissue lysates

WB Recommended dilution:

500-1000