兔抗MRPS35多克隆抗体

Background:

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has had confusing nomenclature in the literature. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 3p, 5q, and 10q.

Applications:

ELISA, WB, IHC

Name of antibody:

MRPS35

Immunogen:

Fusion protein of human MRPS35

Full name:

mitochondrial ribosomal protein S35

Synonyms:

MDS023; MRPS28; MRP-S28; HDCMD11P

SwissProt:

P82673

ELISA Recommended dilution:

5000-10000

IHC positive control:

Human liver cancer

IHC Recommend dilution:

50-200

WB Predicted band size:

37 kDa

WB Positive control:

LOVO, K562, Jurkat and Hela cell lysates

WB Recommended dilution:

500-2000