兔抗PTEN(Phospho-Ser370)多克隆抗体

Background:

This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway.

Applications:

WB, IHC, IF

Name of antibody:

PTEN(Phospho-Ser370)

Immunogen:

Peptide sequence around phosphorylation site of serine 370(D-V-S(p)-D-N) derived from Human PTEN.

Full name:

phosphatase and tensin homolog

Synonyms：

0

SwissProt:

P60484

IHC positive control:

Human breast carcinoma tissue

IHC Recommend dilution:

50-100

WB Predicted band size:

47 kDa

WB Positive control:

Hela cells lysate

WB Recommended dilution:

500-1000

IF positive control:

MCF7 cells

IF Recommend dilution:

100-200